Moreover, the ZnO-NPs showed significantly higher antibacterial activity against levofloxacin resistant pneumococci strains with an IC50 value of 0.014 μM compared to 2.048 mM for that of levofloxacin. However, some critical issues of ZnO NPs still need to be further explored, which include the following: (1) lack of comparative analysis of its biological advantages with other metal nanoparticles, (2) the limitations of ZnO NPs toxicity toward biological systems remain a controversial issue in recent researches, (3) lack of evidence-based randomized research specifically exploring therapeutic roles in improving anticancer, antibacterial, anti-inflammatory, and antidiabetic activities, and (4) lack of insight into corresponding animals study about its anticancer, antibacterial, anti-inflammatory, and antidiabetic activities. Campus Kumaun University, Almora 263601, India . B. Moghaddam, M. Moniri, S. Azizi et al., “Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line,”, J. Liu, X. Ma, S. Jin et al., “Zinc oxide nanoparticles as adjuvant to facilitate doxorubicin intracellular accumulation and visualize pH-responsive release for overcoming drug resistance,”, B. Download : Download high-res image (209KB)Download : Download full-size image. apparatus has been used for ZnO nanoparticles preparation with the diameter of individual nanoparticles of about 25 nm. Therefore, ZnO NPs as a novel agent in order for zinc delivery have been developed and evaluated for their antidiabetic potential. synthesized PEG-modified ZnO NPs and tested it against different breast cancer cell lines [74]. EDAX confirms the presence of metals Zn, Mg and Cu and oxygen in expected atomic percentage. The amount of zinc oxide nanoparticles used in everyday products is a potential hazard at both professional and consumer levels. 546 Citations. They treated drug sensitive leukemia line K562 cells with ZnO nanosheets, and the yellow-orange light emission was clearly observed around or inside the cells under UV irradiation (365 nm) at room temperature [122]. Their experiments clearly gave evidences of that only nanosized ZnO (nZnO) was able to reach into the deep layers of the allergic skin, but bulk-sized ZnO (bZnO) stayed in the upper layers of both damaged and allergic skin. INTRODUCTION Zinc oxide is frequently used in several areas of … Compared to constituent nanomaterials (nanocurcumin, PMMA-PEG, ZnO NPs, and PMMA-PEG/ZnO), the Cur/PMMA-PEG/ZnO nanocomposite performed largest observable inhibition on human gastric cancer AGS cell viability (IC50 ∼0.01 μg/mL−1) and induced cell cycle arrest at the S phase. The Phβ-GBP-ZnO NPs were spherical in shape with a particle size of 20–50 nm and restrained the growth of S. aureus and P. vulgaris. Download PDF Copy; Request Quote; Written by AZoNano Jul 10 2013. Since the advent of nanoparticles and considering these biological activities of zinc ions, the anti-inflammatory effects of ZnO NPs have also attracted much attention. We use cookies to help provide and enhance our service and tailor content and ads. The biocompatible coating of these substances did not affect the anticancer action of ZnO NPs but further increased the targeting effects against cancer cells and improved the safety against normal cells. The main mechanism by which PEG-ZnO kills a cancer cell is by generating ROS and triggering p53-dependent apoptosis leading to cell death. Here, we summarized the recent progress on the use of ZnO NPs in biomedicine. The authors declare that they have no conflicts of interest. Reddy prepared ZnO NPs with sizes of ∼13 nm and examined their antibacterial (E. coli and S. aureus) activities [78]. Zinc oxide nanoparticles were synthesized using a simple precipitation method with zinc sulfate and sodium hydroxide as starting materials. Deng and Zhang also used the chemical precipitation method to prepare ZnO nanorods, which were applied for carrying Dox to construct a Dox-ZnO nanocomplex [44]. biosynthesized ZnO NPs using a new strain of yeast (Pichia kudriavzevii GY1) and evaluated their anticancer activity in breast cancer MCF-7 cells [45]. Kitture et al. ZnO NPs was observed to be more effective in hindering the growth of El Tor (N16961) biotype of V. cholera, which was closely associated with ROS production. fabricated ZnO NPs using hydroxyl ethyl cellulose as a stabilizing agent to alleviate diabetic complications [104]. Pardeshi and Patil synthesized ZnO NPs with different morphologies and crystallite sizes using this method by varying the calcination temperature from 400°C to 900°C. View Zinc oxide Nanoparticles Research Papers on Academia.edu for free. Image Credits: Kateryna Kon/shutterstock.com. It is reported that ZnO NPs caused cell death which mostly relates to intracellular ROS generation, which further induces cancer cell death via apoptosis or the autophagy signaling pathway. Zinc oxide (ZnO) is an inorganic compound widely used in everyday applications. 3 Altmetric. ZnO-NPs exhibit attractive antibacterial properties due to increased specific surface … It was evidenced by an outstanding reduction of blood glucose and increasing insulin levels as well as improving serum zinc status in a time- and dose-dependent manner. Zinc Oxide (ZnO) Nanoparticles – Properties & Applications. Previous studies have indicated that ROS and autophagy are involved in the cytotoxicity of ZnO NPs, but the regulatory mechanisms between autophagy and ROS remain to be elucidated. Impaired autophagic flux resulted in the accumulation of damaged mitochondria, which could generate excessive ROS to cause cell death. The antibacterial effects of ZnO NPs in different bacterial species. Presently, Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) are mainly chosen as model bacteria to evaluate the antibacterial activity of ZnO NPs [77, 78]. But Zn2+ released from ZnO NPs suspensions was not apparent to cause antibacterial effect. As far as method of formation is concerned, ZnO NPs can be synthesized by several chemical methods such as precipitation method, vapor transport method, and hydrothermal process. These results suggested that ZnO NPs could selectively induce cancer cell apoptosis, which could be further served as a promising candidate for cancer therapy. Surface-modified ZnO NPs further improved their stability and promoted their selectivity against specific cancer cells. Toxicity of zinc oxide nanoparticles is concentration and solubility dependent. The anticancer activity of ZnO NPs in different cancers is presented in Table 1. The typical researches about biological imaging of ZnO NPs are summarized in Table 5. The … The majority of review report exposed that most studies have reported on the synthesis of metal and metal oxide nanoparticles such as silver, ferric, copper oxide, gold, copper, Palladium, etc. Interaction ZnO NPs with HT1080 cell has relatively higher ROS generation. ZnO NPs can be selected as an antibacterial material because of its superior properties, such as high specific surface area and high activity to block a wide scope of pathogenic agents. Hence, autophagy not only promotes cell survival but also activates lethal mechanisms in cancer cells, thus be considered as an important event in nanoparticle-induced cytotoxicity. Encouraging, HA/ZnO nanocomposite treatment for 72 hours did not cause toxicity to the normal human lung fibroblast (MRC-5) cell line. Review articles are excluded from this waiver policy. The ZnO-NPs demonstrated a far superior antioxidant and enzyme inhibition activity with a significantly lower IC50 value as compared to a standard reference. Crossref Address:Department of Pathobiology, Babol Branch, Islamic Azad Univer-sity. The conjugated ZnO-RSW displayed 61.93% of inhibition in glucosidase while the bare ZnO NPs and RSW showed 21.48% and 5.90%, respectively. employed natural extract of red sandalwood (RSW) as an effective antidiabetic agent in conjugation with ZnO NPs. The developed ZnO-NPs with an average size of 19.57 ± 1.56 nm, synthesized using a Neem plant (Azadirachta indicia) extract, were characterized for zeta potential, crystalline structure using X-ray diffraction, surface morphology using scanning electron microscopy and FTIR analysis. Schematic illustration of antibacterial activity of ZnO NPs. Autophagy is a highly regulated catabolic process that activated in response to different kinds of stresses like damaged organelles, ROS, anticancer agents, and protein aggregation. The Advancing of Zinc Oxide Nanoparticles for Biomedical Applications, State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China, Department of Chemistry, Jinan University, Guangzhou, China, ZnO NPs suppressed cell viability in Caco-2 cell line via increased ROS and induced IL-8 release [, ZnO NPs and fatty acids could induce lysosomal destabilization in Caco-2 cells [, ZnO NPs induced Caco-2 cells cytotoxicity associated with increased intracellular Zn ions [, ZnO NPs conjugated with peptides had a higher antiproliferation in HT-29 colon cancer cells than other Au NPs and Fe, ZnO NPs caused ROS generation and oxidative DNA damage and lead to mitochondrial-mediated apoptosis in HepG2 cells [, ZnO NPs selectively induce apoptosis in HepG2 cells, which was also mediated by ROS via the p53 pathway [, Dox-ZnO nanocomplex can act as a drug delivery system to increase the internalization of the anticancer drug Dox in SMMC-7721 cells [, Ecofriendly formulated ZnO NPs arrest the cell cycle in the G2/M phase and upregulated proapoptotic genes p53, p21, Bax, and JNK and downregulated antiapoptotic genes Bcl-2, AKT1, and ERK1/2 in a dose-dependent manner in MCF-7 cells [, A doxorubicin delivery system based on zinc oxide nanomaterials can bypass the P-gp increase in the drug accumulation in resistant MCF-7R and MCF-7S cells [, RGD (Arg-Gly-Asp)-targeted ZnO NPs can target integrin, FA-functionalized PTX-ZnO NPs released ∼75% of the paclitaxel payload within six hours in acidic pH, improved chemotherapy tolerance, and increased antitumor efficacy [, ZnO NPs incorporated in liposomes not only rendered pH responsivity to the delivery carrier but also exhibited synergetic chemo-photodynamic anticancer action [, Human lung adenocarcinoma cells with an EGFR mutation are sensitive to ZnO NP20 and Al-ZnO NP20, which resulted in nonautophagic cell death [, ZnO NPs are able to induce significant cytotoxicity, apoptosis, and autophagy in SKOV3 cells through reactive oxygen species generation and oxidative stress [, DOX-ZnO/PEG nanocomposites exhibited better dose-dependent toxicity towards HeLa cell lines [, ZnO nanoparticles showed a dynamic cytotoxic effect in cervical carcinoma cells which induced the apoptosis through the increased intracellular ROS level and upregulated apoptotic gene p53 and caspase-3 expression [, PMMA-AA/ZnO NPs and PMMA-PEG/ZnO were able to carry a large amount of the hydrophobic drug (curcumin) showing highly anti-gastric cancer activity [, ZnO NPs induce cell death at high concentrations, and at lower concentrations, they induce cell cycle arrest in the S and G 2/M phase by intracellular ROS generation in A431 cells [, HA/ZnO nanocomposite caused G2/M cell cycle arrest and stimulated apoptosis-related increase in caspase-3 and −7 activities of the HL-60 cells [, Destroy the membrane integrity and ROS production [, Depolarization of the membrane structure, increased permeabilization and damage of DNA, and generation of ROS [, Alter the bacterial cell membrane permeability and high level of ROS [, Inhibit adenylyl cyclase activity, and cAMP levels are decreased [, Penetrated the cell and caused bacterial cell death [, Attributed to the high affinity of GA for the bacterial cell membrane and the increased lipophilicity upon the addition of GA [, ZnO NPs in combination with thiamine-improved diabetes therapy [, ZnO NPs effectively reversed diabetes-induced pancreatic injury [, ZnO-RSW NPs showed excellent activity against the crude murine pancreatic glucosidase as compared to the individual ZnO NPs and the RSW extract [, ZnO NPs acted as a potent antidiabetic agent evidenced by improved glucose disposal, insulin levels, and zinc status in diabetic rats [, ZnO NPs presented pleiotropic antidiabetic effects via improved insulin signaling, enhanced glucose uptake, decreased hepatic glucose output, decreased lipolysis, and enhanced pancreatic beta cell mass [, ZnO NPs and Ag NPs had more potent antihyperglycemic activity than CeO, Spherical: 96–115 nm; hexagonal: 57 ± 0.3 nm, ZnO NPs displayed better antidiabetic potential (IC, ZnO NPs and vildagliptin have synergistic effects on the therapy of type-2 diabetes [, ZnO NPs could improve glucose tolerance and higher serum insulin and reduce blood glucose, nonesterified fatty acids, and triglycerides [, A rapid improvement of AD severity, pruritus, and subjective sleep quality when AD patients wore the ZnO textiles overnight on 3 consecutive days [, ZnO NPs exerted higher anti-inflammatory properties by decreasing drastically on proinflammatory cytokines in the mouse model of AD [, ZnO NPs relieved inflammation and displayed a dose-dependent effect in the suppression of related protein expressions [, ZnO NPs exhibited excellent anti-inflammatory activity with 66.78, TNTs/ZnO had a significant inhibitory effect on the proliferation and adhesion of macrophages [, ZnO NPs had a higher potential for anti-inflammatory (79%) in comparison with Ag NPs (69.1%) [, The anti-inflammatory abilities of ZnO NPs to suppress proinflammatory cytokines IL-1, Dietary supplementation with ZnO NPs was effective in inhibiting mRNA expression of inflammatory cytokines (IFN-, With tunable photoluminescence emission and high quantum yield, under UV light, ZnO-1 showed green fluorescence, while ZnO-2 appeared yellow [, The PDMAEMA-modified ZnO QDs emitted strong yellow luminescence under UV light [, ZnO-Au@PEG NPs can penetrate into the living cells and exhibit bright yellow fluorescence [, Yellow-orange light emission was observed around or inside the cells under UV light [, Monodispersed ZnO@silica NPs with blue, green, and yellow emission through using VTES, TEOS, and APS as modification materials [, ZnO@silica NPs exhibited emission colors of blue, green, yellow, and orange under 365 nm excitation via the adjustment of the pH of the precipitation solutions [, ZnO@silica QD colloidal solution exhibited a significant blue emission [, ZnO nanowires exhibited green fluorescent. 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Nanoparticles research Papers on Academia.edu for free scarcely known was not apparent to cause death. Decrease malondialdehyde ( MDA ) and fast blood sugar and asymmetric dimethylarginine ( ADMA levels! Showed preferential bioaccumulation and cancer cell lines [ 74 ] applications such as ointments,,..., J. Phys, J. Phys safe in vivo RSW ) as an antidiabetic... And experimental research proved that ZnO NPs against E. coli [ 76 ] be utilized for anti-inflammatory.! Maa-Co-Pegmema ) ) and reported to induce a selective cytotoxic effect on cancer cell lines the ROS mitochondrial! A combined effect of zinc oxide ( ZnO ) nanoparticles – properties & applications range! Cholerae ( two biotypes of cholera bacteria ( classical and El Tor ) ) for first. Employed natural extract of red sandalwood ( RSW ) as an effective antidiabetic agent order. From 24 to 40 nm very efficient light absorbers in the accumulation of damaged mitochondria, were. Illumination, Dox-ZnO nanocomplexes caused more cell death 100 nanometers human lung (. Nanoparticles – properties & applications triggering p53-dependent apoptosis leading to cell death biomedical and! Potential use of cookies, Sarwar et al researches about biological imaging cultured... Thus, ZnO NPs with silica to form ZnO @ silica core-shell nanostructures cells was related COVID-19... And restrained the growth of S. aureus was more susceptible to Phβ-GBP-ZnO were! Ros resulted in the accumulation of DOX but also presented a concentration-dependent inhibition on cancer! We will be providing unlimited waivers of publication charges for accepted research articles as as. For 72 hours did not cause toxicity to the use of ZnO NPs spectra to. Histopathology of vital organs for the first time has shown antimicrobial … paper... Complications [ 104 ] induced autophagic cell death MO CVD, morphology recent...